Ion channels are expressed in virtually all cells of the body. They are large, transmembrane proteins and it is notoriously difficult to obtain sufficient amounts of channel protein for expression or structural studies. We have optimized a protocol for expression and purification of human ion channel proteins from yeast cells. (Scharff-Poulsen and Pedersen, 2013; Tejada et al., 2017a)
The aim of this project is to characterize the functional properties of the purified proteins and determine whether they behave as native proteins. The purified proteins are reconstituted into lipid bilayers and single-channel currents are recorded, and the project can easily be expanded depending on interests and results can be compared to native currents or channels expressed in mammalian cells or Xenopus laevis oocytes (Tejada et al., 2017b), based on your own recordings or literature studies.
Research Institution: University of Copenhagen Faculty of Health and Medical Sciences, Department of Veterinary and Animal Sciences